LEITZ DIALUX 20 - Microscopen-specialist.nl PDF

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LEITZ DIALUX 20LEITZ means precision worldwide.Laboratoryand Research MicroscopeLeitz instruments are guaranteed for two yearsagainst manufacturing defects when purchasedInstructionsthrough an authorized dealer and registeredwith E. Leitz, Inc. Rockleigh, N. J., within 10days of receipt. A registration card is enclosedwith each instruntent for this purpose." Registered TrademarkDes ign subject to alterations without notice.ERNST LEITZ Wri:TZLAR GMBHD-6330 Wetz lar Tel. : (06441) 291Telex : 483 84'3 leiz dSubsidiary : Erns! Leitz (Canada) Ltd ., Midland, On tarioLeitz Portugal S.A.R.L., Vi la Nova de FamalicaoListI 512-150/ Eng I. IPr inted in W-G ermanyI/77/ GX/SD512-150/Engl.I

LEITZ DIALUX 20Laboratoryand Research MicroscopeInstructions4Technical descriptionTechnical detailsTubesRevolving nosepieceMechanical StageCoarse and fine adjustmentLamp Housing 102 ZCondensersObjectivesEyepiecesAssembling the microscopeCentring the lampPreparing the microscope for operation56Centring the condenserOil immersionTransmitted-light darkgroundPhase contrastMicroscopic measurementsPolarizationTransmitted light fluorescenceIncident-light fluorescenceCare and maintenanceAccessories1232Page3 and 3866677891213141719202222232728283237395

1 Technical descriptionFig . 1DI AL UX 20 wit h Lamp Hou si ng 102 Z, UK universalcondenser , Mechanica l Stage No. 78 and FSAphototube.1, 2 Knurled knobs for lamp adjustment3 Lamp Housing 102 Z4 Lamp conde nser adj ustme nt5 Rotary knob for th e fine adj ustm ent of theobject stage6 Rota ry knob for the coa rse verti cal adjustmentof th e object stage7 Stand8 FSA phototube9 Eyepiece tube with PERIPLAN " eyepiece10 Filter slot11 Revol ving nos epiece fo r 5 objectives12 NPL FLUOTAR"' objectives13 Mecha ni cal Stage No. 7814 UK universal condense r15 Vertical adjustment of the co ndenser16 Controls for the mecha ni ca l adj ustment of theobject stage17 Adjustable condenser stop18 Fi eld diaphragm12913:;;o.,.,.-------14 "'---- 153

2 Technical detailsTubes Fig 2Binocular tub e S -c;- 6Fig. 5Larg e Mechanical Stage No. 78Binocular observation tube with adjustable eyepiece tubes for the mechanical compensation of the tube lengthwith interpupillary distance adjustment.Tube factor 1xThe mechanical stage measures 200 x100mm, and has coaxial controls forobject movement within an area of50 x 76mm.Fig 3Binocular phototube FSAFig . 6Condens er holderBinocular observation- and phototubewith automatic interpupillary distancecompensation.- 100% of the light enters the eyepiecesL 50% of the light enters the eyepieces, 50 % the phototube10% of the light enters the eyepieces, 90 % the phototubeTube factor 1xdovetailThisverticallyadjustablechanger has centring screws for thecondenser and an adjustable verticalstop (arrow).,. - . : :.22951 · 5 12 R22639 -5 13 R22654 -5 13 R-"'-.-----,--.::lFig . 4Revol ving nosepieceFig. 7Coarse and fine adjustment of the obj ect stageThe revolving nosepiece takes up to 5objectives.Tube lens factor 1x and 1.25x respectively.The coarse and fine adjustment, arranged on both sides of the stand, adjustment range 35mm, actuates the object stage. One interval on the fineadjustment corresponds to a verticaldifference of 2 ,um.22955-51 2 R7

Fig . 8Lamp Hou sing 102 ZCondensersThe Lamp Housing 102 Z acceptstungsten halogen lamps, ultra-highpressure mercury lamps, and high-pressure xenon lamps of up to 100 W. Lampand mirror are centred separately.The lamp condenser can be horizontallyadjusted .12345622656 · 514 A22655·513 RFig. 10SK sta nd ard c ond ense rFig . 9Lamp mount w ith 20 W tun gsten halogen lamp forth e DI ALU X 20 EB .22962 · 514 R81 In terchangea bl e co nd ense r top2 Co nde nse r top ho ld er3 Lever fo r sw in g in g o ut the con d enser to p. Atthe sa me tim e the le ns 6 is sw un g into th ei ll umin ating bea m ;4 Apertur e di aphrag m lever5 Dove tai l change r6 Suppl ementary lensCondenserwithdovetailchange r,swing-out condenser to holder (1 0.2)and supplementary lens (10 .6) . Can beadapted for special purposes with theuse of different condenser tops (10.1)to be screwed into the holder.Fi g. 11UK uni ve rsa l co nde nse r1 Two cen trin g sc rews (o nl y one visib le) for th eli g ht rin g adju stme nt2 In te rchangea bl e co nd ense r top3 Rota tin g turret4 A pertur e di aphrag m5 Dovetail changer6 Suppl ementary lensCondenser with dovetail changer (11 .5) ,swing-out condenser top holder, andsupplementary lens (11 .6), can beadapted for special purposes. Rotaryturrets (11.3) for phase contrast andinterference contrast T can be inserted .9

. Condenser tops for the SK standard condenser and the UK universal condenserCondensertopUsesCondensertopcondenser top turned out(supplementary lens turned in)For objectives of apertures 0.250.90 8 1.1condenser top turned in(supplementary lens turned out)Fo r objectives of apertureOil1 .32condenser top turned in(supplementary lens turnedout) ; immersion oil onfront lens of the condenserFor use with the Oil100/ 1.32objective0.90 81.1UK Universal condenser for phase contrast 0.90 8 1.10.70 8 4condenser top turned in(supplementary lens turned out)Intercept distance 4mm. Forexamination of specimensmounted on microscope slidesthicker than 1 mm .0.55 8 15condenser top turned in(supplementary lens turned out)Intercept distance 15mm. Forexamination of specimens onmicroscope slides thicker than6mm0.35 8 30condenser top turned in(supplementary lens turned out)Intercept distance 30mm . Forexamination of specimens onmicroscope slides thicker than10mmD 0.80condenser top turned in(supplementary lens turned out)Darkground; for objectives ofapertures 0.75D 1.19condenser top turned insupplementary lens turned out) ;immersion oil on front lensof the condenser topDarkground ; for objectives ofaperture 1.10Objectivesengraved:-H12345(all objectives)Phaco 1Phaco 2Phaco 3Phaco 4(all objectives)Aperture 0.75BrightfieldPhase contrastPhase contrastPhase contrastPhase contrastDarkgroundH1234(all objectives)Phaco 1Phaco 2Phaco 3Phaco 4BrightfieldPhase contrastPhase contrastPhase contrastPhase contrastH124(all objectives)Phaco 1Phaco 2Phaco 4BrightfieldPhase contrastPhase contrastPhase contrastH12(all objectives)Phaco 1Phaco 2BrightfieldPhase contrastPhase contrast18 42843844840.55 8 15-1 8 152 8154 8150.35 8 3010Rotaryturretposition1 8 1.12 8 1.13 8 1.14 8 1.1OF 8 8 4Light ring-1 8 302 8 3011

EyepiecesObjectives1 !gl!l!2-JW.,.,.:.;::t;;j3: ; .:.,5 -. Reproductionratio1.6:125:14:16.3:1Col ourgreybrownredorange4s-. --- -.10:116 :125 :140:163 :1100 :1yellowbrightgreendarkgree nbrightbluedarkbluewhite7 -s ;;,.;;:;::;::;:::;:;::22954-519 R22952-519 RFig . 13PERIPLAN GF 10x/18Fig . 12NPL FLUOTAR 100/ 1.32 OILAll LEITZ objectives computed fortube length 160, as well as objectivesof primary magnification greater than16:1 computed for tube length 170(note the engraved values) can be usedon the DIALUX 20.The following data are engraved on theobjective mounts:1 160 (170) : mechanical tube length.The distance in mm between theshoulder of the objective and therim of the tube.2 0.17: coverglass thickness . Onlyspecimensunder a coverglass(0 .17mm thickness) should be observed with these objectives. If thefigure 0.17 is replaced by a dash(-) specimens may be observedwith or without a coverglass .3 Normal piano objectives (flattenedfield of view of up to at least 18mmintermediate image).Piano objectives (flattened field ofview of up to 28mm iveshave no additional letters engraved .Objectives for phase contrast observation have the additional designation Phaco (all the NPL-FLUOTAR objectives are engraved ingreen throughout) and the ind icationof the associated position of thePhaco ring turret of the UK universal condenser (e .g. Phaco 1 turret position 1).Reproduction ratio: The dimensionalratio of intermediate image and object (e.g. 100:1).Numerical aperture : (e .g. /1.32).The aperture indication is followedby that of the immersion medium.Colour code see above table.Immersion objectives have an additional black ring oil immersionor white ring water immersion.LEITZ eyepieces computed for 160mmmechanical tube length are used. Theydiffer from conventional eyepieces inthe additional indication of the field-ofview index following that of the magnification.ditional TL 160 distance ring (Fig. 14)must be inserted.The field-of-view of an eyepiece is thearea of the intermediate image in thetube that can be observed in it itappears magnified by the eyepiecefactor.The diameter of the image producedby a GF 10x/ 18 eyepiece therefore appears as large as that of an area10 x 18 180mm at a distance of250mm from the observer.If the diameter of the field of view isdivided by the objective magnification(and any tube factor involved) (turret1x, PLOEMOPAK 1.25x) the diameterof the object area observed is obtained.With the previously mentioned GF 1Ox/18 eyepiece and a 25/ 0.55 objective anobject area of18x2522970-512 RFi g. 14Inse rtin g th e di stanc e ring TL 160 (arrow)1 0.72mm diametercan therefore be observed. The finalmagnification of the microscope isbased on the following formula:Reproduction ratio of the objective xeyepiece magnification (x tube factor) .Example:Objective 25/0.55Eyepiece 1Ox/18Tube factor 1xTotal magnification 25 x 10 x 1 250:1"If LEITZ eyepieces of conventionaldesign (without indication of the fieldof-view index) are to be used, an ad-13

3 Assemb l ing the microscopeFig. 15In se rting th e tubeFig. 18In se rting the condenserPress the lever for tube change in thedirection of the arrow and drop the tubeinto the bayonet changer. Allow thelever to return to the front. After it islocked, the tube can be rotated through360 .lnsertthe eyepieces in their tubes .Lower the condenser dovetai l guidewith knob (18 .1) unti l the condensercan be easi ly inserted in it as far as itwill go. T he aperture d iaphragm lever(18.2) must face the observer. Ra isethe condenser fully.1- 512 AFig . 16Screwing in the objectives22956-514 AFig. 19Removing the transport anchorageScrew the objectives into the apertureson the revolving nosepi ece at increasing magnification (e .g. 6.3, 16, 40 etc.).Attention: before inserting the lamphousing remove the transport anchoring screw (arrow) of the lamp condenser on the bottom of the lamp housing.Fi g . 17Ins ert in g the revo lvin g nosepi ec e w ith ob jectivesFig. 20Attaching the Lamp Housi ng 102 ZLower the object stage with the coarseadjustment (1 .6) , release the clampingscrew and full y insert the revolvingnosepiece with the objectives in thehorizontal dovetail guide; tighten theclamping screw.Set the bayonet lever (20.1) of the lamphous ing vertica ll y. Insert the changingcollar of the lamp housing in thebayonet fitting of the microscope andsecure it.tO22636-512 A1415

Centring the lamp(Lamp Housing 102 Z)Changing the lampRelease the knurled screw (24.7) of thelamp mount. Pull the lamp mount (24.8)out of the lamp housing . Remove thedefective lamp. Insert the new tungstenhalogen lamp before removing its protective cover (21.2) . Replace the lampmount (21 .1) and centre the lamp (Fig.25) .Fully open the field diaphragm (1.18) .Insert the adjustment device in the filterslot (Fig. 23).Turn the microscope sideways foreasier access to all centring screws ofthe lamp housing.22656·5 14 AFig . 23Adjustment device (with the diagrammatically represented lamp adjustment).Fig. 21Inserting the tun gsten halogen lampFig. 25Image of the lamp filament and its mirror im age(diagrammatic representation)Fig. 24Lamp Housing 102 ZI I 29229-514 AForm a sharp im ageof the lamp filamentby turning the lampco nd ense r knob (2).29230-514 AMove the lamp filamentimage into the upperhalf of the illuminatedfield wit h the centringscrew (1).22637·514 A29231-514 AAdjust the lampfi lame nt image withcentring screw (3) soth at it occ upi es theupp er illuminatedarea co mpletely.29232·514 ABy ad justin g the twoce ntri ng screws (4, 6)capture the mirrorimage of th e lam pfilament in the lowe rhalf of th e illuminated area .Fig . 22In serting the mou nt wit h lamp29233-514 AAdjust the mirrorhorizontall y (5) sothat the mirror imageof the lamp fil amentalso appears in sharpfoc us.1629234-514ASet the mirror im agewith the two ad justment screws 4 and 6so that it coincideswith the primary imageof the lamp filamentin the lower half.29235·514 ASet the two imagesof the lamp filamentwith the fin e adju stment of the lamp (1)and mirror adjust mentscrews (4) so thattheir sides exactlycoincide.Th e two im agessho uld now justco in cide in thece ntre.17

4 preparing the microscope for operationChanging the Lamps in the DIALUX 20 EBSw itch on the microscope illumination.Mount the specimen with object holderon the ob ject stage.The c lamping of the specimen is adjustab le: pu sh the button (Fig. 29,arrow) on the pivot of the spec imenholder, push it to the left for firmer, tothe right for less firm clamping and letit cl ick in position.For initial observation choose an objective of medium magnification (e.g .NPL FLUOTAR 16/ 0.40).Give the screw (1 .17) about 5 anticlockwise turns and fully raise the condenserwith knurled screw (1.15).Turn the condenser top in. Open theaperture diaphragm (10/ 11.4) and thefield diaphragm (1.18).When the binocular observation tube S(Fig . 30) is used, carry out interpupillary distance adjustment (by pulling orpushing) so that both images are coincident (only one circular image isseen). Transfer the interpupillary distance determined (scale on the frontplate of the tube) to the two eyepiecetubes - e.g. with a 65mm interpupillardistance set the two eyepiece tubeseach at their " 65" mark (Fig. 31).Carry out the fo ll owing corrections fordefective vision:Look through the right-hand eyepiecewith your right eye and focus the specimen w ith the fine adjustment. Nowlook at the same area of the specimenthrough the left-h and eyepiece withyour left eye and aga in focus the specimen by rotating the left-hand eyepiece tube - do not use the fine adjustment. Check this setting finally aftercentration of the condenser.Fig . 2923181-514R23022-5 12 AFi g. 30Fi g. 26Pull ing out th e EB lamp moun t.Fig. 28In se rting th e lamp mountPull the lamp mount out of the fo ot ofthe stand. Remove the defective lam pIn sert new tungsten halogen lamp together with its protective cover, whi chshould be removed only after inserti on .Replace the lamp mount in the foo t ofthe stand.liEFig. 31Fig . 27Insert ing a new lamp.22972-512 A1819

Centring the condenserFocus the specimen with the coarseand fine adjustment.Adjust the field diaphragm so th at thewhite ring (Fig. 32, arrow) is com pletelycovered .1. Close the field diaphragm.2. By rotating the condenser stop sc rewlower the condenser so that the edg eof the field diaphragm appears sharp.3. Centre the image of the field di aphragm with the two centring sc rews.4. Open the fiel

4 Aperture diaphrag m 5 Dovetail changer 6 Supplementary lens Condenser with dovetail changer (11 .5) , swing-out condenser top holder, and supplementary lens (11 .6), can be adapted for special purposes. Rotary turrets (11.3) for phase contrast and interference contrast T can be inserted. 9