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Analyzing Single-Molecule Time Series via Nonparametric ...

Analyzing Single-Molecule Time Series via Nonparametric ...

Analyzing Single-Molecule Time Series via Nonparametric Bayesian Inference Keegan E. Hines,1 John R. Bankston,2 and Richard W. Aldrich1,* 1Center for Learning and Memory and Department of Neuroscience, University of Texas at Austin, Austin, Texas; and 2Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, Washington ABSTRACT The ability to measure the ...

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Supplemental Material for: Analyzing Single Molecule Time ...

Supplemental Material for: Analyzing Single Molecule Time ...

Supplemental Material for: Analyzing Single Molecule Time Series Using Nonparametric Bayesian Inference Keegan E. Hines1, John R. Bankston2, and Richard W. Aldrich1 1Center for Learning and Memory and Department of Neuroscience ,The University of Texas at Austin, Austin,TX,78712 2Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, WA, 98195 1 ...

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Analyzing single-molecule manipulation experiments

Analyzing single-molecule manipulation experiments

In this paper we demonstrate how time domain based computational modeling techniques utilizing local maximum likelihood methods (Calderon, 2007a; Calderon and Chelli, 2008; Calderon et al., 2009a,b) can be used to extract kinetic information from single-molecule time series where a molecule isstretchedbyanexternalforce.Specifically,weuseanAFMtipto

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Analyzing Single-Molecule Protein Transportation ...

Analyzing Single-Molecule Protein Transportation ...

Analyzing Single-Molecule Protein Transportation Experiments via Hierarchical Hidden Markov Models Yang Chen , Kuang Shen , Shu-Ou Shan , S. C. Kou This revision December 2015 Abstract To maintain proper cellular functions, over 50% of proteins encoded in the genome need to be transported to cellular membranes. The molecular mechanism behind such a process, often referred to as protein ...

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SMART: Single Molecule Analysis Research Tool Version 1

SMART: Single Molecule Analysis Research Tool Version 1

The Single Molecule Analysis Research Tool (SMART) package provides a compre-hensive set of tools for analyzing single molecule data. This instruction manual aims to provide a detailed account of all the functionality built into SMART so that users can get the most from the package. To aid in presenting these topics video tutorials have been recorded to further supplement this manual. To Begin ...

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Step Detection in Single-Molecule Real Time Trajectories ...

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[36,37]. As we show, colored noise is present in single-molecule real time trajectories collected from optical tweezers. The assumption of Gaussian white noise for single-molecule data that contains colored noise can result in significant fitting errors. It is thus critical to assess the structure of the noise when analyzing these single-molecule trajectories. We have developed a statistical ...

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Workflow for processing high throughput Single Molecule ...

Circular-consensus, Single Molecule, Real-Time (SMRT) Sequencing reads in the 1-3 kb range, with >99% accuracy can be generated using the previous generation PacBio RS II or, in much higher throughput, using the new Sequel System. While throughput is lower compared to short-read sequencing methods, the reads are a true random sampling of the underlying community since SMRT Sequencing has been ...

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for analyzing gene regulation. Herein, we will review these single-molecule 5mC and 5hmC quantitative methods. 2 NANOPORE SEQUENCING As a promising next-generation sequencing technique, nanopore sequencing provides the possibility of single-molecule detection without the needs for sample labeling or amplification.16 In 1996, this nanopore-based technique was first mentioned by Deamer and ...

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Single-molecule PCR: an artifact-free PCR approach for the ...

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single-molecule PCR is a simple alternative that promises to evade the disadvantages inherent to post-PCR cloning and enhance mutational analysis in the future. Expert Rev. Mol. Diagn. 5(5), xxx–xxx (2005) Somatic mutations are responsible for develop-mental defects, cancer and possibly aging. To fully appreciate the abundance and impact of somatic mutations, and to study their sources, one ...

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Single-molecule analysis of fluorescently labeled G-protein ...

Single-molecule analysis of fluorescently labeled G-protein ...

Single-molecule analysis of fluorescently labeled G-protein–coupled receptors reveals complexes with distinct dynamics and organization Davide Calebiroa,b,1, Finn Rieken a,b, Julia Wagner , Titiwat Sungkaworna,b,c, Ulrike Zabela,b,Alfio Borzid, Emanuele Cocuccie, Alexander Zürna, and Martin J. Lohsea,b,1 aInstitute of Pharmacology and Toxicology and bRudolf Virchow Center, Deutsche ...

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ANALYSIS OF NEUTRON SMALL ANGLE SCATTERING DATA FOR ...

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molécule par un grand nombre de petites sphères superposées remplissant l'espace occupé par la molécule. On décrit une technique permettant de faire correspondre à l'essai la fonction de diffusion élargie par résolution du modèle en faisant varier les paramètres choisis et facteurs de normalisation du modèle afin d'obtenir le meilleur accord ( X2 minimum). On calcule le rayon de ...

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