State-Dependent accessibility of the P-S6 linker of ...
and S357C, but not M358C and HCN1-R, were sensitive to Cd2? blockade (IC 50 = 3–12 lM vs. [1 mM). External application of the positively charged covalent sulfhydryl modi?er MTSET irreversibly reduced I-140mV of Q353C and A354C to 27.9 ± 3.4% and 58.2 ± 13.1% of the control, respectively, and caused signi?cant steady-state activation shifts (DV 1/2 = –21.1 ± 1.6 for Q353C and-10.0 ...
Continue Reading...